NIKON A1R雙光子熒光顯微鏡

生產公司👐🏿👨🏽‍⚖️：NIKON（日本）

儀器型號🚲🤙：A1R MP

主要性能指標：

1、可見光激光器部分🕴：

Ar離子激光器: 波長488nm;

綠光源激光器: 波長543nm🫴🏽；

紅光源激光器：波 長640nm

2、紅外雙光子飛秒激光器，波長 690-1040nm,

3、掃描系統：線掃描速度15000線/秒；掃描速度：512 X 512條件下30幅/秒🙍🏼‍♀️，共聚焦點掃描分辨率: 4096 X 4096像數點。

4、物鏡：全部配置APO共聚焦顯微鏡專用高分辨率、高透過率物鏡, 配置全套 DIC棱鏡

16倍APO共聚焦專用物鏡，數值孔徑0.80

25倍 APO 共聚焦專用水鏡💻，數值孔徑1.10,

工作距離 2.0 mm

40倍 APO 共聚焦專用水鏡,數值孔徑 0.8

60倍 APO 共聚焦專用水鏡,數值孔徑 1.1

適用範圍💯：

1、細胞間通訊的研究；

2、細胞物理化學測定；

3💟、多點瞬間光活化光刺激功能；

4、電生理研究✶🌺；

      5、pH、細胞內離子分析👨🏿‍💻；

      6、在體活體觀察等；

儀器功能特色：

1、光刺激和圖像掃描能同步觸發、同步記錄🤰👫，並能進行電生理信號和共聚焦圖像信號的疊加。提供光刺激和掃描事件的同步TTL輸出；

2☆❤️‍、掃描速度：512 X 512條件下30幅/秒🪺；

      3、利用共振掃描器，實現雙光子刺激（光遺傳激活,FRAP,FLIP，uncage等）的同時進行單光子video成像🏢。

      4🚹、具有紅外飛秒激光自動準直功能🧍‍♂️。

放置地點：上海市華山路1954號意昂4平台教三樓Med-X研究院117室

聯系人：顧翔

電話：13764512572 

Email：Hallelujah@sjtu.edu.cn

Nikon A1R Multiphoton Fluorescence Confocal Microscope

Manufacturing Company: NIKON (Japan)

Instrument Model: A1R MP

Main performance index:

1. The part of visible light laser:

2. Ar ionic laser: wavelength 488nm;

3. Green light source laser: wavelength 543nm;

4. Red source laser: wavelength 640nm

5. Infrared two-photon femtosecond laser: wavelength 690-1040nm,

6. Scanning system: the speed of line scanning is 15000 lines/second;

7. Scanning speed: 30 images/second under the condition of 512 X 512 pixels，confocal scanning resolution: 4096 X 4096 pixels.

8. Objective lenses: All APO confocal microscope is equipped with special high resolution and high transmittance objectives lenses, and a full set of DIC prisms.

16X APO confocal objective lens, numerical aperture 0.80

25X APO confocal objective lens, numerical aperture 1.10,

Working distance 2.0mm

40X APO confocal special water objective lens, numerical aperture 0.8

60X APO confocal special water objective lens, numerical aperture 1.1

Sphere of application:

1. The study of intercellular communication;

2. Physical and chemical determination of cells;

3. The function of multi-point instantaneous photo-activation and photo-stimulation;

4. Electrophysiological research;

5. pH and intercellular ionic analysis;

6. In vivo and viviperception observation, etc.

Instrument features:

1. Light stimulation and image scanning can trigger and record synchronously, and signals of electrophysiological and confocal image can be superimposed. This instrument also can provide synchronous TTL output of light stimulation and scan events;

2. Scanning speed: 30 images/second under the condition of 512 X 512 pixels;

3. Multiphoton stimulation and the image formation of single photon video can be realized simultaneously (optogenetic activation, FRAP, FLIP, UNCAGE, etc.) using resonance scanner.

4. Possessing the automatic calibration function of infrared femtosecond laser.

Location: Room 117, MED-X Research Institute, No.3 Teaching Building, Shanghai Jiaotong University, No. 1954 Huashan Road, Shanghai

Contact: Shawn Gu

Contact Information:

Telephone: 13764512572

Email: Hallelujah@sjtu.edu.cn